• Background

  Quite a lot of attention has during the last years been paid to the health risk associated with microplastics in the environment and in food. However, the assessments done are so far inconclusive, and the Norwegian Scientific Committee for Food and Environment affirms that still very limited data of acceptable quality are available on levels of microplastics in foods. In PLASTPATH, we will study if it is reasonable to claim that microplastic in the environment play an important role in the dissemination and protection of bacteria and virus transmitted via food and water. In case, it means that abundance of microplastics will contribute to increased numbers of food- and waterborne diseases. Our hypothesis is that bacteria and virus, by hitch-hiking attached to the microplastics within the food production chains, will travel longer and be more protected compared to the microbes that “travel alone”. We assume that this microplastic protection is relevant until pathogenic microbes are released in the gut or being absorbed into body tissues.

• Objective

  The project will start by characterization of microbe populations on the surface of microplastics isolated from wastewater and sludge from two wastewater treatment plants and fresh water (Lierelva) environments. Further we will study of how such microbe populations develop in controlled laboratory experiments by using standard cultivation procedures and a novel microfluidic platform. The next steps are investigations of the impact of the microbe-microplastic association on microbial survival through an in vitro digestive system and on the pathogenicity of the microbes and the interaction of the plastisphere in the digestive system of Medaka fish in an in vivo model.

Participants

External participants